EMBOSS explorer

restrict

Report restriction enzyme cleavage sites in a nucleotide sequence (read the manual)

Unshaded fields are optional and can safely be ignored. (hide optional fields)

Input section

Select an input sequence. Use one of the following three fields:

  1. To access a sequence from a database, enter the USA here:
  2. To upload a sequence from your local computer, select it here:
  3. To enter the sequence data manually, type here:

Restriction enzyme data file (optional). Use one of the following two fields:

  1. To access a standard EMBOSS data file, enter the name here:
  2. To upload a data file from your local computer, select it here:

Restriction enzyme methylation data file (optional). Use one of the following two fields:

  1. To access a standard EMBOSS data file, enter the name here:
  2. To upload a data file from your local computer, select it here:
Required section
Minimum recognition site length
Comma separated enzyme list
Advanced section
Minimum cuts per RE
Maximum cuts per RE
List individual enzymes with their fragments?
Force single site only cuts?
Allow blunt end cutters?
Allow sticky end cutters?
Allow ambiguous matches?
Allow circular DNA?
Use methylation data?
Only enzymes with suppliers?
Output section
Limits reports to one isoschizomer?
Sort output alphabetically?
Show fragment lengths?
Show sequence name?
Output report format
Run section
Email address: If you are submitting a long job and would like to be informed by email when it finishes, enter your email address here.